|
| Status |
Public on Jan 26, 2011 |
| Title |
WBC1 |
| Sample type |
genomic |
| |
|
| Source name |
White blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: White blood cells
|
| Treatment protocol |
All samples were bisulfite modified using kits from Qiagen and Sigma
|
| Growth protocol |
Placenta samples were stored in RNAlater prior to DNA extraction, WBC samples are collected in ACD tubes, AnCHMs and MCT DNA were extracted from snap frozen tissues.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extraction was performed using standard protocols.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina protocol for DNA methylation.
|
| |
|
| Hybridization protocol |
Standard Illumina protocol for DNA methylation.
|
| Scan protocol |
Standard Illumina protocol for DNA methylation using the Bead Array Scanner.
|
| Description |
White blood cells 1
|
| Data processing |
The data was generated using the BeadStudio 2.0 software provided by Illumina by following their analytical matrix. Average normalization was performed.
|
| |
|
| Submission date |
Jun 02, 2010 |
| Last update date |
Jan 26, 2011 |
| Contact name |
Rosanna Weksberg |
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics and Genome Biology
|
| Lab |
Weksberg Lab
|
| Street address |
555 University Ave.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE22091 |
A Novel Approach Identifies New Differentially Methylated Regions (DMRs) Associated with Imprinted Genes |
|
