|
Status |
Public on Aug 18, 2022 |
Title |
CT13 |
Sample type |
SRA |
|
|
Source name |
AB1-HA tumor
|
Organism |
Mus musculus |
Characteristics |
tissue: tumor strain: BALB/c age: 8-10 weeks gender: m response: CR timepoint: Day-1
|
Treatment protocol |
200 mg/kg cyclophosphamide on day 10. Surgery was performed to excise analysed tumor on day 9, day 13 or day 17.
|
Growth protocol |
subcutaneous tumor growth
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen tumour dissociation with Trizol followed by chloroform extraction and purification using RNeasy MinElute Illumina 50bp single-end sequencing
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
read alignment with Kallisto count quantification with tximport Genome_build: GRCm38.p6 Supplementary_files_format_and_content: count matrix with counts generated from txi import on kallisto aligned samples
|
|
|
Submission date |
Aug 24, 2021 |
Last update date |
Aug 18, 2022 |
Contact name |
Caitlin M Tilsed |
Organization name |
NCARD,UWA
|
Street address |
6 Verdun St
|
City |
Nedlands |
State/province |
WA |
ZIP/Postal code |
6009 |
Country |
Australia |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE182674 |
Bulk RNAseq of AB1-HA murine mesothelioma tumors from complete responders and partial responders to cyclophosphamide chemotherapy |
|
Relations |
BioSample |
SAMN20960104 |
SRA |
SRX11895931 |