|
| Status |
Public on Jul 18, 2024 |
| Title |
G: GE-0123 VS GE-0135 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Untreated glioblastoma multiforme specimen 2
|
| Organism |
Homo sapiens |
| Characteristics |
age: 81
Sex: Male
|
| Treatment protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
| Growth protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
|
| Label |
Cy5
|
| Label protocol |
Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
|
| |
|
| Channel 2 |
| Source name |
Untreated glioblastoma multiforme specimen 3
|
| Organism |
Homo sapiens |
| Characteristics |
age: 69
Sex: Female
|
| Treatment protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
| Growth protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
|
| Label |
Cy3
|
| Label protocol |
Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
|
| |
|
| |
| Hybridization protocol |
Microarrays were hybridized and washed according to manufacturer's protocol (version 6.5)
|
| Scan protocol |
Scanned on an Agilent G2505C scanner
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
|
| |
|
| Submission date |
Aug 24, 2021 |
| Last update date |
Jul 18, 2024 |
| Contact name |
Víctor M. Barberá |
| E-mail(s) |
barbera_vicjua@gva.es
|
| Phone |
+34966616127
|
| Organization name |
Hospital General Universitario de Elche
|
| Department |
Laboratorio de Genética Molecular
|
| Street address |
Camí de l'Almazara, 11
|
| City |
Elche |
| State/province |
Not US or Canada |
| ZIP/Postal code |
03203 |
| Country |
Spain |
| |
|
| Platform ID |
GPL30538 |
| Series (1) |
| GSE182697 |
Transcriptomic study in human glioblastoma multiforme by DNA microarray |
|
