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Sample GSM5534813 Query DataSets for GSM5534813
Status Public on Jul 18, 2024
Title I: GE-0135 VS GE-0123
Sample type RNA
 
Channel 1
Source name Untreated glioblastoma multiforme specimen 3
Organism Homo sapiens
Characteristics age: 69
Sex: Female
Treatment protocol The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
Growth protocol The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
Extracted molecule total RNA
Extraction protocol Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
Label Cy5
Label protocol Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
 
Channel 2
Source name Untreated glioblastoma multiforme specimen 2
Organism Homo sapiens
Characteristics age: 81
Sex: Male
Treatment protocol The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
Growth protocol The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
Extracted molecule total RNA
Extraction protocol Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
Label Cy3
Label protocol Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
 
 
Hybridization protocol Microarrays were hybridized and washed according to manufacturer's protocol (version 6.5)
Scan protocol Scanned on an Agilent G2505C scanner
Data processing Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
 
Submission date Aug 24, 2021
Last update date Jul 18, 2024
Contact name Víctor M. Barberá
E-mail(s) barbera_vicjua@gva.es
Phone +34966616127
Organization name Hospital General Universitario de Elche
Department Laboratorio de Genética Molecular
Street address Camí de l'Almazara, 11
City Elche
State/province Not US or Canada
ZIP/Postal code 03203
Country Spain
 
Platform ID GPL30538
Series (1)
GSE182697 Transcriptomic study in human glioblastoma multiforme by DNA microarray

Data table header descriptions
ID_REF
VALUE Background corrected and normalized with loess and Aquantile log2 ratio (Cy5/Cy3)

Data table
ID_REF VALUE
1 0.380976824
2 -0.117582659
3 -0.090834217
4 -0.584299444
5 0.182554526
6 0.141815491
7 0.048041931
8 0.090354272
9 -0.820621873
10 -0.058141354
11 -0.039493868
12 0.181497333
13 0.336961346
14 0.877518372
15 0.275181529
16 -0.080024377
17 -0.180444093
18 0.06760649
19 -3.360019347
20 -0.094144457

Total number of rows: 15744

Table truncated, full table size 271 Kbytes.




Supplementary file Size Download File type/resource
GSM5534813_I.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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