|
Status |
Public on Jul 18, 2024 |
Title |
I: GE-0135 VS GE-0123 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Untreated glioblastoma multiforme specimen 3
|
Organism |
Homo sapiens |
Characteristics |
age: 69 Sex: Female
|
Treatment protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
Growth protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
|
Label |
Cy5
|
Label protocol |
Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
|
|
|
Channel 2 |
Source name |
Untreated glioblastoma multiforme specimen 2
|
Organism |
Homo sapiens |
Characteristics |
age: 81 Sex: Male
|
Treatment protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
Growth protocol |
The samples were not cultured or treated in cell culture, they were processed directly from the surgical resection specimen of the tumor.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA isolation from human GBM biopsies was performed using the RNAqueous-Micro Kit (Ambion), following the manufacturer’s instructions. Normal human brain RNA was purchased from BioChain
|
Label |
Cy3
|
Label protocol |
Two-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling (version 6.5)
|
|
|
|
Hybridization protocol |
Microarrays were hybridized and washed according to manufacturer's protocol (version 6.5)
|
Scan protocol |
Scanned on an Agilent G2505C scanner
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
|
|
|
Submission date |
Aug 24, 2021 |
Last update date |
Jul 18, 2024 |
Contact name |
Víctor M. Barberá |
E-mail(s) |
barbera_vicjua@gva.es
|
Phone |
+34966616127
|
Organization name |
Hospital General Universitario de Elche
|
Department |
Laboratorio de Genética Molecular
|
Street address |
Camí de l'Almazara, 11
|
City |
Elche |
State/province |
Not US or Canada |
ZIP/Postal code |
03203 |
Country |
Spain |
|
|
Platform ID |
GPL30538 |
Series (1) |
GSE182697 |
Transcriptomic study in human glioblastoma multiforme by DNA microarray |
|