|
| Status |
Public on Aug 29, 2021 |
| Title |
GDT_plus_IL2 |
| Sample type |
SRA |
| |
|
| Source name |
gammadelta T cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell type: gammadelta T cells
treatment: IL-2 expanded
|
| Treatment protocol |
Gammadelta T cells expanded with IL-2 [100U/ml] for 5 days.
|
| Growth protocol |
Freshly sorted by FACS murine gammadelta T cells were activated with plate-bound anti-CD3 and soluble anti-CD28 antibodies in the presence of recombinant mouse IL-2 [100U/ml].
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured cells using the RNeasy Mini kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina bcl2fastq v2.18.0.12 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using STAR 2.6.0.a with default parameters.
The HTSeq software (v.0.6.0) was applied to generate the count matrix, with default parameters. Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using EdgeR 3.28.1.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: read_counts.txt: Matrix table with raw gene counts for every gene and every sample.
|
| |
|
| Submission date |
Aug 26, 2021 |
| Last update date |
Aug 30, 2021 |
| Contact name |
Bin Zhang |
| Organization name |
City of Hope
|
| Department |
Hem Malignancies Trans Sci
|
| Street address |
1218 S 5TH AVE
|
| City |
Monrovia |
| State/province |
CA |
| ZIP/Postal code |
91016 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE182882 |
Isolation and expansion of murine γδ T cells from mouse splenocytes |
|
| Relations |
| BioSample |
SAMN21011818 |
| SRA |
SRX11931978 |
