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Sample GSM5550721 Query DataSets for GSM5550721
Status Public on Apr 30, 2023
Title vehicle, 24 hours, biological rep1
Sample type RNA
 
Source name HTR8/Svneo trophoblast cells
Organism Homo sapiens
Characteristics tissue: trophoblast cells
genotype: wildtype
agent: vehicle
Treatment protocol For coumestrol treatment, HTR8/Svneo cells were plated onto a 6 well plate using RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin. Upon reaching confluency, cells were treated with media (RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin) containing vehicle or coumestrol (65┬ÁM) for 24 hours.
Growth protocol HTR8/Svneo cells were grown and maintained with RPMI-1640 with 5% fetal bovine serum and 1% penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Cells were washed with sterile PBS. RNA was extracted using the Ambion mirVana miRNA isolation kit with phenol for isolation of total RNA according to manufacturer's instructions (Thermo fisher Scientific #AM1560)
Label gene expression data from vehicle treated, 24 hour time point, HTR8/Svneo Trophoblast cells
Label protocol Biotinylated cRNA was prepared according to Affymetrix protocol.
 
Hybridization protocol GeneChips were washed and stained in the 2 GeneChip Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader
Data processing Data was analyzed using Partek Genomics Suite with Gene Expression Workflow. The microarray data was analyzed using the RMA Import Settings on Partek Genomics Suite 7.0. The default RMA settings include "Interrogating Probes" to import, with no probe filtering, with RMA Background Correction, Quantile Normalization, a Log Probes using Base number 2, with a Median Polish Probeset Summarization.
 
Submission date Aug 31, 2021
Last update date Apr 30, 2023
Contact name Kathleen M Caron
E-mail(s) kathleen_caron@med.unc.edu
Organization name University of North Carolin
Department Cell Biology and Physiology
Street address 111 Mason Farm Road, CB7545
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL23159
Series (1)
GSE183072 Gene Expression Changes after Coumestrol Stimulation of Human Trophoblast Cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
TC0100006437.hg.1 5.16689
TC0100006476.hg.1 7.23787
TC0100006479.hg.1 7.9673
TC0100006480.hg.1 6.59387
TC0100006483.hg.1 6.01457
TC0100006486.hg.1 8.59941
TC0100006490.hg.1 5.94536
TC0100006492.hg.1 6.27535
TC0100006494.hg.1 6.84485
TC0100006497.hg.1 6.27818
TC0100006499.hg.1 8.05833
TC0100006501.hg.1 7.15967
TC0100006502.hg.1 5.1936
TC0100006514.hg.1 6.11325
TC0100006516.hg.1 7.46945
TC0100006517.hg.1 5.19789
TC0100006524.hg.1 7.2737
TC0100006540.hg.1 5.5311
TC0100006548.hg.1 4.72233
TC0100006550.hg.1 8.66231

Total number of rows: 21448

Table truncated, full table size 545 Kbytes.




Supplementary file Size Download File type/resource
GSM5550721_veh_24hr_1.CEL.gz 1.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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