|
| Status |
Public on Apr 30, 2023 |
| Title |
vehicle, 24 hours, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HTR8/Svneo trophoblast cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: trophoblast cells
genotype: wildtype
agent: vehicle
|
| Treatment protocol |
For coumestrol treatment, HTR8/Svneo cells were plated onto a 6 well plate using RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin. Upon reaching confluency, cells were treated with media (RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin) containing vehicle or coumestrol (65µM) for 24 hours.
|
| Growth protocol |
HTR8/Svneo cells were grown and maintained with RPMI-1640 with 5% fetal bovine serum and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed with sterile PBS. RNA was extracted using the Ambion mirVana miRNA isolation kit with phenol for isolation of total RNA according to manufacturer's instructions (Thermo fisher Scientific #AM1560)
|
| Label |
gene expression data from vehicle treated, 24 hour time point, HTR8/Svneo Trophoblast cells
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix protocol.
|
| |
|
| Hybridization protocol |
GeneChips were washed and stained in the 2 GeneChip Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader
|
| Data processing |
Data was analyzed using Partek Genomics Suite with Gene Expression Workflow. The microarray data was analyzed using the RMA Import Settings on Partek Genomics Suite 7.0. The default RMA settings include "Interrogating Probes" to import, with no probe filtering, with RMA Background Correction, Quantile Normalization, a Log Probes using Base number 2, with a Median Polish Probeset Summarization.
|
| |
|
| Submission date |
Aug 31, 2021 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Kathleen M Caron |
| E-mail(s) |
kathleen_caron@med.unc.edu
|
| Organization name |
University of North Carolin
|
| Department |
Cell Biology and Physiology
|
| Street address |
111 Mason Farm Road, CB7545
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE183072 |
Gene Expression Changes after Coumestrol Stimulation of Human Trophoblast Cells |
|
