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Sample GSM5550726 Query DataSets for GSM5550726
Status Public on Apr 30, 2023
Title coumestrol, 24 hours, biological rep3
Sample type RNA
Source name HTR8/Svneo trophoblast cells
Organism Homo sapiens
Characteristics tissue: trophoblast cells
genotype: wildtype
agent: coumestrol
Treatment protocol For coumestrol treatment, HTR8/Svneo cells were plated onto a 6 well plate using RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin. Upon reaching confluency, cells were treated with media (RPMI-1640 without phenol red with 5% fetal bovine serum and 1% penicillin/streptomycin) containing vehicle or coumestrol (65µM) for 24 hours.
Growth protocol HTR8/Svneo cells were grown and maintained with RPMI-1640 with 5% fetal bovine serum and 1% penicillin/streptomycin.
Extracted molecule total RNA
Extraction protocol Cells were washed with sterile PBS. RNA was extracted using the Ambion mirVana miRNA isolation kit with phenol for isolation of total RNA according to manufacturer's instructions (Thermo fisher Scientific #AM1560)
Label gene expression data from coumestrol treated (65µM), 24 hour time point, HTR8/Svneo Trophoblast cells
Label protocol Biotinylated cRNA was prepared according to Affymetrix protocol.
Hybridization protocol GeneChips were washed and stained in the 2 GeneChip Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader
Data processing Data was analyzed using Partek Genomics Suite with Gene Expression Workflow. The microarray data was analyzed using the RMA Import Settings on Partek Genomics Suite 7.0. The default RMA settings include "Interrogating Probes" to import, with no probe filtering, with RMA Background Correction, Quantile Normalization, a Log Probes using Base number 2, with a Median Polish Probeset Summarization.
Submission date Aug 31, 2021
Last update date Apr 30, 2023
Contact name Kathleen M Caron
Organization name University of North Carolin
Department Cell Biology and Physiology
Street address 111 Mason Farm Road, CB7545
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platform ID GPL23159
Series (1)
GSE183072 Gene Expression Changes after Coumestrol Stimulation of Human Trophoblast Cells

Data table header descriptions
VALUE normalized signal intensity

Data table
TC0100006437.hg.1 5.28762
TC0100006476.hg.1 7.33518
TC0100006479.hg.1 7.56953
TC0100006480.hg.1 6.80633
TC0100006483.hg.1 5.77611
TC0100006486.hg.1 8.51014
TC0100006490.hg.1 5.72946
TC0100006492.hg.1 6.24004
TC0100006494.hg.1 6.87928
TC0100006497.hg.1 5.7778
TC0100006499.hg.1 7.62012
TC0100006501.hg.1 7.08304
TC0100006502.hg.1 5.14893
TC0100006514.hg.1 5.933
TC0100006516.hg.1 7.45277
TC0100006517.hg.1 5.45446
TC0100006524.hg.1 7.1328
TC0100006540.hg.1 5.78935
TC0100006548.hg.1 5.04266
TC0100006550.hg.1 8.50563

Total number of rows: 21448

Table truncated, full table size 545 Kbytes.

Supplementary file Size Download File type/resource
GSM5550726_cou_24hr_3.CEL.gz 1.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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