|
Status |
Public on Dec 31, 2021 |
Title |
L1701735: kd_5-1_light_5d |
Sample type |
SRA |
|
|
Source name |
Seedling
|
Organism |
Arabidopsis thaliana |
Characteristics |
age: 5 days after germination genotype: snrk1a1/a2 light: light treatment: - tissue: seedling
|
Extracted molecule |
total RNA |
Extraction protocol |
Seedlings were harvested, frozen in liquid nitrogen and RNA was harvested using QIAGEN RNEasy Micro-Kit. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Sequenced reads were mapped to TAIR10 by STAR for the whole genome using featurecount. Analysis were performed with DESeq2 Genome_build: TAIR10 Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
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|
|
Submission date |
Aug 31, 2021 |
Last update date |
Dec 31, 2021 |
Contact name |
Markus Henninger |
Organization name |
University of Würzburg
|
Street address |
Julius-von-Sachs Platz 2
|
City |
Würzburg |
ZIP/Postal code |
97084 |
Country |
Germany |
|
|
Platform ID |
GPL19580 |
Series (1) |
GSE183118 |
Next Generation Sequencing Facillitates Quantitative Analysis of Wild Type and snrk1a1/a2 mutant Transcriptomes |
|
Relations |
BioSample |
SAMN21160048 |
SRA |
SRX11975591 |