|
Status |
Public on Sep 07, 2024 |
Title |
WTK2 |
Sample type |
SRA |
|
|
Source name |
mice primary bone marrow-derived macrophages
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J treatment: Treated with B16F10 TCM + KCl
|
Treatment protocol |
Primary BMDMs from C57B6J mice were treated with B16F10 tumor cells conditioned medium supplemented with/without KCl or NaCl for 6 hours, and the final KCl and NaCl concentration was 40mM.
|
Growth protocol |
BMDMs were cultured with DMEM supplemented with 10% FBS, 1% AA.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with RNA extraction kit following manufacturer's instructions RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Raw reads were processed through quality control and genome mapping and further analyzed by the DESeq2 package (1.30.1). Differential expressed genes were determined with a cutoff of FDR smaller than 0.05. Normalized transcripts per million values (TPM) were used to plot heatmap using pheatmap package (1.0.12). none provided by the submitter
|
|
|
Submission date |
Sep 07, 2021 |
Last update date |
Sep 07, 2024 |
Contact name |
Di Wang |
E-mail(s) |
diwang@zju.edu.cn
|
Organization name |
Zhejiang University
|
Department |
School of Medicine
|
Lab |
Immunometabolism Lab
|
Street address |
Yuhgang Tang Rd No.866
|
City |
Hangzhou |
State/province |
Zhejiang |
ZIP/Postal code |
310058 |
Country |
China |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE183634 |
High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after tumor supernatants induction |
|
Relations |
BioSample |
SAMN21335665 |
SRA |
SRX12067745 |