|
| Status |
Public on Aug 03, 2010 |
| Title |
Karyotyping of EC haploid strains (S. cerevisiae) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
EC9-7 haploid strain
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: EC9-7 haploid strain (JYL2155), the haploid segregant of EC9
|
| Growth protocol |
Grow in YPD medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
| Label |
Cy5
|
| Label protocol |
The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| |
|
| Channel 2 |
| Source name |
w303 haploid strain, control
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: w303 haploid strain (JYL1127)
|
| Growth protocol |
Grow in YPD medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
| Label |
Cy3
|
| Label protocol |
The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| |
|
| |
| Hybridization protocol |
The hybridization protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| Scan protocol |
Scanned on an Molecular Device AXON 4000B (version GenePix 6.0) and got .gpr files
|
| Description |
The karyotype of EC9 haploid segregants(EC9-5) which was analyzed by array-CGH to identify amplified chromosomal regions.
|
| Data processing |
Subtracted background. Dividing by ch2 (control) was used to transform data (Agilent GeneSpring 7.3.1 software)
|
| |
|
| Submission date |
Jun 18, 2010 |
| Last update date |
Aug 03, 2010 |
| Contact name |
Jun-Yi Leu |
| E-mail(s) |
jleu@imb.sinica.edu.tw
|
| Organization name |
Academia Sinica
|
| Department |
Insititute of Molecular Biolody
|
| Street address |
128 Academia Road, Section 2, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL7305 |
| Series (1) |
| GSE22431 |
Characterization of chromosome rearrangement of EC strain (S. cerevisiae) |
|
