|
| Status |
Public on Nov 16, 2023 |
| Title |
16HBE ex14 + HGF [257236334397_S01_GE1_107_Sep09_1_3] |
| Sample type |
RNA |
| |
|
| Source name |
16HBE ex14 + HGF
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 16HBE
genotype: ex14
agent: HGF
|
| Treatment protocol |
16HBE WT and 16HBE ex14 were starved (0% FBS) during 2 hours and next stimulated 24 hours by HGF (20 ng/ml)
|
| Growth protocol |
MEM, 10% FBS and 1% Zell-Shield, 37°C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
manufacturer's instructions
|
| |
|
| Hybridization protocol |
Agilent-072363 Whole Human Genome Microarray 8x60 v3 were scanned on an Agilent G2505B scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version V.10.7.3.1).
|
| Description |
ex14 treated by HGF
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 21, 2021 |
| Last update date |
Nov 16, 2023 |
| Contact name |
frédéric leprêtre |
| E-mail(s) |
frederic.lepretre@univ-lille.fr
|
| Organization name |
Lille University
|
| Department |
CHU of Lille, CBP
|
| Lab |
plateforme de génomique fonctionnelle et structurale
|
| Street address |
Boulevard du Pr Leclercq
|
| City |
lille |
| ZIP/Postal code |
59037 |
| Country |
France |
| |
|
| Platform ID |
GPL20844 |
| Series (1) |
| GSE184514 |
Trancriptome profiling of genome-edited 16HBE cells on the receptor tyrosine kinase MET in response to HGF |
|
