|
Status |
Public on Nov 16, 2023 |
Title |
16HBE WT [257236334397_S01_GE1_107_Sep09_2_1] |
Sample type |
RNA |
|
|
Source name |
16HBE WT
|
Organism |
Homo sapiens |
Characteristics |
cell line: 16HBE genotype: WT agent: none
|
Treatment protocol |
16HBE WT and 16HBE ex14 were starved (0% FBS) during 2 hours and next stimulated 24 hours by HGF (20 ng/ml)
|
Growth protocol |
MEM, 10% FBS and 1% Zell-Shield, 37°C, 5% CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
manufacturer's instructions
|
|
|
Hybridization protocol |
Agilent-072363 Whole Human Genome Microarray 8x60 v3 were scanned on an Agilent G2505B scanner.
|
Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version V.10.7.3.1).
|
Description |
WT non treated
|
Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Sep 21, 2021 |
Last update date |
Nov 16, 2023 |
Contact name |
frédéric leprêtre |
E-mail(s) |
frederic.lepretre@univ-lille.fr
|
Organization name |
Lille University
|
Department |
CHU of Lille, CBP
|
Lab |
plateforme de génomique fonctionnelle et structurale
|
Street address |
Boulevard du Pr Leclercq
|
City |
lille |
ZIP/Postal code |
59037 |
Country |
France |
|
|
Platform ID |
GPL20844 |
Series (1) |
GSE184514 |
Trancriptome profiling of genome-edited 16HBE cells on the receptor tyrosine kinase MET in response to HGF |
|