|
| Status |
Public on Jun 01, 2022 |
| Title |
Th1_cultured_DO.11_expt1 |
| Sample type |
RNA |
| |
|
| Source name |
cultured DO.11 T cells
|
| Organism |
Mus musculus |
| Characteristics |
pre vs post injection: Pre
cle disease first vs flare: N/A
th skewing: Th1
mouse cle skin disease score: N/A
|
| Growth protocol |
For cultures, DO.11 T cells were isolated from donor spleens, activated with ova-pulsed splenocytes, skewed with cytokines and antibodies towards Th0, Th1 or Th2, and expanded with IL-2 for a total of 10 days (see also Mande et al JCI 2018 PMID: 29889098). For ex vivo analysis, DO.11 T cells were enriched from digested lesional skin tissue using Miltenyi CD3 bead isolation kits.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from T cells using Qiagen RNEasy mini kits per the manufacturer's instructions.
|
| Label |
fluorescent probe
|
| Label protocol |
Probe labeling was performed per NanoString manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridizations were performed per NanoString manufacturer's protocol overnight for 18h in a BioRad C1000 touch thermocycler
|
| Scan protocol |
Cartridges were run on a NanoString Sprint machine.
|
| Description |
Th1 GVH35 setup
|
| Data processing |
Data were analyzed with nSolver software version 4.0 and with ROSALIND software OnRamp Bio.
|
| |
|
| Submission date |
Oct 05, 2021 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Jillian M Richmond |
| E-mail(s) |
jillian.richmond@umassmed.edu
|
| Organization name |
UMass Chan Medical School
|
| Department |
Dermatology
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL30820 |
| Series (2) |
| GSE185355 |
Gene expression analysis of skewed mouse T cells pre- vs post-injection, and ex vivo skin T cells from first disease vs flare, in a model of Cutaneous Lupus Erythematosus |
| GSE186096 |
Gene expression and Targeted protein expression analysis of skewed or drug-treated mouse T cells, and of serum from first disease vs flare in a model of Cutaneous Lupus Erythematosus |
|
