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Sample GSM5611800 Query DataSets for GSM5611800
Status Public on Nov 01, 2022
Title MEF_4F_D3
Sample type RNA
Source name mouse embryonic fibroblasts infected with retroviruses carrying Gata4, Hand2, Mef2C, and Tbx5 day 3 post-infection.
Organism Mus musculus
Characteristics cell type: embryonic fibroblasts
infected with: Gata4, Hand2, Mef2C, and Tbx5
time post infection: 3 days
Treatment protocol Cells were infected with retroviruses carrying carrying Gata4, Hand2, Mef2C, and Tbx5 and cultured for various time period.
Growth protocol Cells were cultured in medium composed of DMEM/199 (4:1) (Gibco), 10% FBS (Gemini), 5% horse serum (Gemini), antibiotics (Gibco), 1X non-essential amino acids (Gibco), 1X essential amino acids (Gibco), 1 B-27 (Gibco), 1 insulin–selenium–transferin (Gibco), 1 MEM vitamin solution (Gibco) and 1X sodium pyruvate (Gibco). The medium was changed every 2–3 days until cells were harvested for RNA extraction.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRizol reagent (Ambion) and the RNeasy Plus Universal Mini Kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5.5 ug total RNA (GeneChip WT Plus Reagent Kit, 2013, Affymetrix).
Hybridization protocol Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Cartridge Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description Total RNA extracted from mouse embryonic fibroblasts infected with retroviruses carrying Gata4, Hand2, Mef2C, and Tbx5 day 3 post-infection.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Normalized data file includes MAS5.0 signal intensity
Submission date Oct 05, 2021
Last update date Nov 01, 2022
Contact name Hongyan Xu
Organization name Augusta University
Department Population Health Sciences
Street address 1120 15th St
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
Platform ID GPL6246
Series (1)
GSE185370 Expression data from mouse embryonic fibroblasts

Supplementary data files not provided
Processed data are available on Series record

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