|
| Status |
Public on Jun 01, 2022 |
| Title |
Th1 sup |
| Sample type |
protein |
| |
|
| Source name |
cultured DO.11 T cells
|
| Organism |
Mus musculus |
| Characteristics |
pre vs post injection: Pre
cle disease first vs flare: N/A
th skewing: Th1
mouse cle skin disease score: N/A
|
| Treatment protocol |
For drug treatments, Th0 unskewed DO.11 T cells were cultured in the presence of 100uM hydrochlorothiazide (HCTZ), 1uM 5' fluorouracil (FU), 10uM terbinafine (Ter), or vehicle control (Veh) and ova peptide pulsed APCs for a total of 10 days as for skewing growth protocol.
|
| Growth protocol |
For cultures, DO.11 T cells were isolated from donor spleens, activated with ova-pulsed splenocytes, skewed with cytokines and antibodies towards Th0, Th1 or Th2, and expanded with IL-2 for a total of 10 days (see also Mande et al JCI 2018 PMID: 29889098). For ex vivo analysis, DO.11 T cells were enriched from digested lesional skin tissue using Miltenyi CD3 bead isolation kits. For serum analysis, mice were bled at first disease presentation (approximately week 4) or flare (approximately week 12).
|
| Extracted molecule |
protein |
| Extraction protocol |
Cell culture supernatants or sera were isolated after centrifugation at 330 x g for 5 min and were stored at -70C until used in assays. Samples were prepared per the manufacturer's instructions (RayBiotech).
|
| Label |
fluorescent antibody quantitative detection
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
Binding was performed per RayBiotech manufacturer's protocol
|
| Scan protocol |
Slides were shipped to RayBiotech manufacturer for their in-house Quantibody testing service
|
| Description |
raw data file: slide 1.xlsx
|
| Data processing |
Backgound median fluorescence intensity was subtracted from sample median fluorescence intensity
|
| |
|
| Submission date |
Oct 19, 2021 |
| Last update date |
Jun 01, 2022 |
| Contact name |
Jillian M Richmond |
| E-mail(s) |
jillian.richmond@umassmed.edu
|
| Organization name |
UMass Chan Medical School
|
| Department |
Dermatology
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL30876 |
| Series (2) |
| GSE186095 |
Targeted protein expression analysis of skewed or drug-treated mouse T cells, and of serum from first disease vs flare in a model of Cutaneous Lupus Erythematosus |
| GSE186096 |
Gene expression and Targeted protein expression analysis of skewed or drug-treated mouse T cells, and of serum from first disease vs flare in a model of Cutaneous Lupus Erythematosus |
|
