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Sample GSM5661569 Query DataSets for GSM5661569
Status Public on Oct 31, 2021
Title 4М biological rep 4
Sample type RNA
 
Source name CD14+ monocytes
Organism Homo sapiens
Characteristics disease: control
Treatment protocol Monocytes did not undergo any treatment
Growth protocol Peripheral venous blood samples were collected prior to the caesarian section in EDTA-treated tubes. CD14+ and CD16+ monocytes were isolated using magnetic sorting and immediately lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from patient
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
 
Submission date Oct 29, 2021
Last update date Oct 31, 2021
Contact name Polina Vishnyakova
E-mail(s) vpa2002@mail.ru
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
 
Platform ID GPL16686
Series (1)
GSE186819 Gene expression in CD14+ and CD16+ monocytes

Data table header descriptions
ID_REF
VALUE log2-rma

Data table
ID_REF VALUE
16650001 1.58156
16650003 2.48026
16650005 3.55404
16650007 1.80381
16650009 1.63784
16650011 2.57362
16650013 4.70511
16650015 4.82837
16650017 2.60456
16650019 2.72553
16650021 2.89443
16650023 2.30162
16650025 2.48976
16650027 1.36463
16650029 2.42954
16650031 3.29028
16650033 1.78115
16650035 1.75291
16650037 1.63238
16650041 4.34176

Total number of rows: 53617

Table truncated, full table size 884 Kbytes.




Supplementary file Size Download File type/resource
GSM5661569_4M_HuGene-2_0-st_.CEL.gz 7.7 Mb (ftp)(http) CEL
GSM5661569_4M_HuGene-2_0-st_.rma-gene-full.chp.gz 332.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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