|
| Status |
Public on Oct 31, 2021 |
| Title |
10M biological rep 6 |
| Sample type |
RNA |
| |
|
| Source name |
CD14+ monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
disease: late-onset preeclampsia
|
| Treatment protocol |
Monocytes did not undergo any treatment
|
| Growth protocol |
Peripheral venous blood samples were collected prior to the caesarian section in EDTA-treated tubes. CD14+ and CD16+ monocytes were isolated using magnetic sorting and immediately lysed in QIAZOL reagent for the following RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
|
| Description |
Gene expression data from patient
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
|
| |
|
| Submission date |
Oct 29, 2021 |
| Last update date |
Oct 31, 2021 |
| Contact name |
Polina Vishnyakova |
| E-mail(s) |
vpa2002@mail.ru
|
| Organization name |
National Medical Research Center for Obstetrics, Gynecology and Perinatology
|
| Street address |
Oparina 4
|
| City |
Moscow |
| ZIP/Postal code |
116571 |
| Country |
Russia |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE186819 |
Gene expression in CD14+ and CD16+ monocytes |
|
