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Sample GSM5661578 Query DataSets for GSM5661578
Status Public on Oct 31, 2021
Title 13M biological rep 7
Sample type RNA
Source name CD14+ monocytes
Organism Homo sapiens
Characteristics disease: control
Treatment protocol Monocytes did not undergo any treatment
Growth protocol Peripheral venous blood samples were collected prior to the caesarian section in EDTA-treated tubes. CD14+ and CD16+ monocytes were isolated using magnetic sorting and immediately lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from patient
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
Submission date Oct 29, 2021
Last update date Oct 31, 2021
Contact name Polina Vishnyakova
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
Platform ID GPL16686
Series (1)
GSE186819 Gene expression in CD14+ and CD16+ monocytes

Data table header descriptions
VALUE log2-rma

Data table
16650001 2.74654
16650003 2.13815
16650005 2.46218
16650007 1.74988
16650009 1.78703
16650011 2.87425
16650013 4.40477
16650015 5.17837
16650017 2.57371
16650019 3.60828
16650021 3.36217
16650023 1.99062
16650025 3.45882
16650027 2.38751
16650029 1.97212
16650031 3.40546
16650033 1.3117
16650035 2.04073
16650037 2.07917
16650041 4.48237

Total number of rows: 53617

Table truncated, full table size 884 Kbytes.

Supplementary file Size Download File type/resource
GSM5661578_13M_HuGene-2_0-st_.CEL.gz 7.9 Mb (ftp)(http) CEL
GSM5661578_13M_HuGene-2_0-st_.rma-gene-full.chp.gz 335.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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