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Sample GSM5661582 Query DataSets for GSM5661582
Status Public on Oct 31, 2021
Title 18M biological rep 4
Sample type RNA
 
Source name CD16+ monocytes
Organism Homo sapiens
Characteristics disease: control
Treatment protocol Monocytes did not undergo any treatment
Growth protocol Peripheral venous blood samples were collected prior to the caesarian section in EDTA-treated tubes. CD14+ and CD16+ monocytes were isolated using magnetic sorting and immediately lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from patient
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
 
Submission date Oct 29, 2021
Last update date Oct 31, 2021
Contact name Polina Vishnyakova
E-mail(s) vpa2002@mail.ru
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
 
Platform ID GPL16686
Series (1)
GSE186819 Gene expression in CD14+ and CD16+ monocytes

Data table header descriptions
ID_REF
VALUE log2-rma

Data table
ID_REF VALUE
16650001 1.74128
16650003 1.57464
16650005 3.3994
16650007 1.79491
16650009 1.58695
16650011 1.7637
16650013 4.27903
16650015 5.01502
16650017 2.49316
16650019 1.89812
16650021 3.1019
16650023 1.91573
16650025 2.12587
16650027 2.05956
16650029 2.70846
16650031 3.0462
16650033 2.41956
16650035 2.0897
16650037 2.09051
16650041 3.72216

Total number of rows: 53617

Table truncated, full table size 884 Kbytes.




Supplementary file Size Download File type/resource
GSM5661582_18M_HuGene-2_0-st_.CEL.gz 7.7 Mb (ftp)(http) CEL
GSM5661582_18M_HuGene-2_0-st_.rma-gene-full.chp.gz 335.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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