|
| Status |
Public on Jul 24, 2010 |
| Title |
Human pre-germinal center B cell [09-001] |
| Sample type |
SRA |
| |
|
| Source name |
Human Pre-Germinal Center B cells, Tonsil, FACS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: pre-germinal center B cell
source tissue: tonsil
gender: female
age: 3 years
isolation: fluorescence activated cell sorting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation method: Trizol. Small RNA library preparation: Illumina protocol "Preparing Samples for Analysis of Small RNA" (Part # 11251913 Rev. A)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Cell surface markers used for cell sorting: CD19+IgD+CD38+/intCD77-
|
| Data processing |
Image analysis, base calling, quality filtering: Standard settings of GAPipeline 1.0-1.4 using PhiX control; Fastq files contain 30 nt length reads that passed the chastity filter. Qualities are in phred64 format for all files. Alignments were done with a custom software that used bowtie 0.9.9.3 to find the longest, mismatch-free alignable prefix of each read, trimmed any remaining adapter sequence, and then stored the unique sequence reads and their frequency. This data is given in the data table, which therefore gives frequencies only for alignable reads. For further analysis, the alignments were matched up with annotation to determine where the small RNA reads originated from and to profile miRNA expression in different tissues.
|
| |
|
| Submission date |
Jul 22, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Seolkyoung Jung |
| Organization name |
NIH
|
| Department |
NIAMS
|
| Lab |
biodata mining and discovery section
|
| Street address |
10 Center Dr
|
| City |
bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE23090 |
microRNA expression in human tonsillar B cell populations |
|
| Relations |
| SRA |
SRX023948 |
| BioSample |
SAMN00017489 |
