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Sample GSM5699230 Query DataSets for GSM5699230
Status Public on Nov 27, 2023
Title iWAT cont_4
Sample type RNA
 
Source name inguinal white adipose tissue
Organism Mus musculus
Characteristics tissue: inguinal white adipose tissue
strain: Senp2flox/flox
Treatment protocol Eight week-old mice were fed a high-fat diet, (HFD; 58 kcal% fat w/sucrose, D12331; Research Diets, New Brunswick, NJ, USA) for 12 weeks.
Growth protocol All animals were housed at 22-24°C, with a 12:12-h light-dark cycle and ad libitum access to diet and water.
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated using TRIzol (Invitrogen, MA, USA) according to the manufacturer’s instruction.
Label Cy3
Label protocol Cy3 labeled cRNA was prepared from 0.5 ug total RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNeasy column purification (QIAGEN, Valencia, CA).
 
Hybridization protocol Cy3 labeled cDNA was directly mix with hybridization buffer and Agilent blocking agent. Then, it was hybridized on microarrays for 40h at 65ºC in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent).
Scan protocol The array was scanned using a SureScan Microarray Scanner (Agilent).
Description Gene expression profiles of iWAT obtained from control mouse
Data processing The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction software (version 11.5.1) (Agilent). The log2-intensities of the probes from the arrays were normalized using the quantile normalization method.
 
Submission date Nov 22, 2021
Last update date Nov 27, 2023
Contact name Sehyun Chae
E-mail(s) shchae@kbri.re.kr
Organization name Korea Brain Research Institute
Department Neurovascular Unit Research Group
Street address 61, Cheomdan-ro, Dong-gu
City Daegu
ZIP/Postal code 41062
Country South Korea
 
Platform ID GPL13912
Series (1)
GSE189326 SENP2 suppresses browning of white adipose tissues by de-conjugating SUMO from C/EBPβ

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 12.217
2 5.4093
3 5.1872
4 5.3749
5 6.8436
6 5.3375
7 5.9565
8 11.649
9 5.2643
10 5.0194
11 5.6571
12 5.4428
13 7.7797
14 12.591
15 5.5602
16 5.2263
17 10.414
18 7.5711
19 9.899
20 13.88

Total number of rows: 62976

Table truncated, full table size 782 Kbytes.




Supplementary file Size Download File type/resource
GSM5699230_SG13094295_1_4.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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