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Sample GSM5700444 Query DataSets for GSM5700444
Status Public on Nov 27, 2023
Title e3-2: eWAT_Cont_siC/EBPb_3
Sample type SRA
Source name SVF-derived adipocyte
Organism Mus musculus
Characteristics cell type: SVF-derived adipocyte
tissue: epididymal white adipose tissue
strain: Senp2flox/flox
treatment: siC/EBPbeta
Treatment protocol SVF from control and Senp2-aKO mice was treated with C/EBPβ-specific siRNA.
Growth protocol Stromal vascular fraction (SVF) cells were isolated from the iWAT or eWAT. SVF cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco Life Technologies) supplemented with 10 % FBS, and seeded in 12-well plates. Adipocyte differentiation of confluent SVF cells was induced by treatment with DMI (1 mol/l dexamethasone, 0.5 mmol/l isobutylmethyxanthine and 0.1 mg/ml insulin) in DMEM supplemented with 10 % FBS. Two days after the treatment, cells were maintained in DMEM supplemented with 10 % FBS and 1 μg/ml insulin for additional 5 days.
Extracted molecule polyA RNA
Extraction protocol Total RNAs were isolated using TRIzol (Invitrogen, MA, USA) according to the manufacturer’s instruction.
Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA Seq Kit (NEW ENGLAND BioLabs, Inc., UK).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Data processing Illumina Pipeline (CASAVA) v1.8.2 used for basecalling.
Illumina adapter sequences were removed using the cutadapt software (version 2.7), and the remaining reads sequences were aligned to the mouse genome (GRCm38) using Tophat2 software (version 2.1.1) with the default parameters.
After the alignment, the numbers of reads mapped to the gene features (GTF file of GRCm38.91) were counted using HTseq sofrware. The read counts for the samples in each condition were then normalized using the TMM (trimmed mean of M-values) normalization of the edgeR package.
Genome_build: Mus_musculus.GRCm38
Supplementary_files_format_and_content: tab-delimited text files include TMM-normalized read counts for each sample
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
Submission date Nov 23, 2021
Last update date Nov 27, 2023
Contact name Sehyun Chae
Organization name Korea Brain Research Institute
Department Neurovascular Unit Research Group
Street address 61, Cheomdan-ro, Dong-gu
City Daegu
ZIP/Postal code 41062
Country South Korea
Platform ID GPL21273
Series (1)
GSE189387 SENP2 suppresses browning of white adipose tissues by de-conjugating SUMO from C/EBPβ
BioSample SAMN23405368
SRA SRX13205625

Supplementary file Size Download File type/resource
GSM5700444_e3-2.txt.gz 227.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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