|
| Status |
Public on Jul 08, 2011 |
| Title |
Activated tolerogenic DCs, technical replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
DCs derived from CD14+ monocytes from adult human blood, stimulated with IL10/dexamethason for 24 hr, and for an additional 6 hr with LPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: DCs derived from CD14+ blood monocytes cultured for 6 days in the presence of IL4 and GGM-CSF
stimulus: IL10/dexamethasone 24 hr, then LPS 6hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy column purification
|
| Label |
Biotin
|
| Label protocol |
Biotin labeling using Affymetrix 3' IVT Express Kit, following manufacturer's directions
|
| |
|
| Hybridization protocol |
17 hours at 45 degrees Celsius in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using using Stain cocktails included in the Affymetrix 3' IVT Express Kit
|
| Scan protocol |
GeneChip scanner 3000 7G
|
| Description |
Gene expression data from monocyte-derived DCs stimulated with IL10/dexamethason 24hr, LPS 6hr
|
| Data processing |
GCRMA (in Bioconductor version 2.6.1) using R version 2.10.1 on Mac OS X 10.4.11 (PPC), and the Bioconductor packages gcrma (version 2.18.1) and affy (version 1.24.2).
|
| |
|
| Submission date |
Aug 02, 2010 |
| Last update date |
Jul 08, 2011 |
| Contact name |
Bastiaan Jansen |
| E-mail(s) |
bjhjansen@gmail.com
|
| Organization name |
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Center Nijmegen
|
| Department |
Tumor Immunology
|
| Street address |
PO Box 9101
|
| City |
Nijmegen |
| ZIP/Postal code |
6500 HB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23371 |
Transcriptomes of monocyte-derived DCs stimulated with various compounds |
|
