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Sample GSM573516 Query DataSets for GSM573516
Status Public on Aug 02, 2012
Title small RNAs from vegetative cells_SL140
Sample type SRA
Source name vegetative cells
Organism Chlamydomonas reinhardtii
Characteristics strain: cc124-
Treatment protocol Small RNAs were isolated from different strains of Chlamydomonas and from cells at different phases of the Chlamydomonas sexual cycle. Genomic DNA was isolated from vegetative cells.
Growth protocol Chlamydomonas cells were grown in liquid culture.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Trizol (Invitrogen) and small RNA isolated from the total RNA fraction using mirVana (Ambion), adapter sequences appropriate Illumina sequencing were ligated.
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina Genome Analyzer
Data processing Sequence reads were obtained using the Illumina Genome Analyzer Pipeline. The 3 prime adaptor sequence was removed from the small RNA reads and the reads were aligned to the Chlamydomonas genome, assembly 4. Only reads with 100% match to the genome were retained.
Submission date Aug 02, 2010
Last update date May 15, 2019
Contact name Krys Kelly
Organization name University of Cambridge
Department Plant Sciences
Lab Baulcombe Group
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3EA
Country United Kingdom
Platform ID GPL9152
Series (1)
GSE23379 Small RNA profiling of strains and lifecycle of Chlamydomonas reinhardtii
SRA SRX025304
BioSample SAMN00027522

Supplementary file Size Download File type/resource
GSM573516_SL140.v_Chlamydomonas_reinhardtii_genome.patman.aligned_reads.fasta.txt.gz 583.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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