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Sample GSM573681 Query DataSets for GSM573681
Status Public on Jul 29, 2011
Title mRNA OSE 434
Sample type RNA
 
Source name Ovarian Surface Epithelia
Organism Homo sapiens
Characteristics tumor stage/grade: N/A
age at surgery: 41
Treatment protocol Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
Extracted molecule total RNA
Extraction protocol RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
Label biotin
Label protocol Affymetrix IVT Labeling Kit
 
Hybridization protocol Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
Scan protocol as per manufacturer's protocol using GeneChip Scanner 3000.
Description Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
Data processing MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
 
Submission date Aug 03, 2010
Last update date Jul 29, 2011
Contact name Shubin Shahab
E-mail(s) shubin.shahab@gmail.com
Organization name Georgia Institute of Technology
Department Biology
Lab McDonald
Street address 310 Ferst Drive
City Atlanta
State/province GA
ZIP/Postal code 30332
Country USA
 
Platform ID GPL570
Series (2)
GSE23391 miRNAs in ovarian cancer: A systems approach (mRNA data)
GSE23392 miRNAs in ovarian cancer: A systems approach

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 4097.15 P 0.00110197
AFFX-BioB-M_at 5128.98 P 4.42873e-05
AFFX-BioB-3_at 3892.59 P 4.42873e-05
AFFX-BioC-5_at 7455.09 P 4.42873e-05
AFFX-BioC-3_at 9922.54 P 4.42873e-05
AFFX-BioDn-5_at 18389.9 P 4.42873e-05
AFFX-BioDn-3_at 29763.7 P 0.000224668
AFFX-CreX-5_at 85422.8 P 5.16732e-05
AFFX-CreX-3_at 87602.1 P 4.42873e-05
AFFX-DapX-5_at 12.1415 A 0.868639
AFFX-DapX-M_at 23.0547 A 0.852061
AFFX-DapX-3_at 15.1153 A 0.574038
AFFX-LysX-5_at 127.432 A 0.327079
AFFX-LysX-M_at 87.1478 A 0.287743
AFFX-LysX-3_at 16.0452 A 0.953518
AFFX-PheX-5_at 8.50825 A 0.966323
AFFX-PheX-M_at 18.7805 A 0.804734
AFFX-PheX-3_at 138.316 A 0.287743
AFFX-ThrX-5_at 147.05 A 0.300606
AFFX-ThrX-M_at 52.9294 A 0.699394

Total number of rows: 54675

Table truncated, full table size 1607 Kbytes.




Supplementary file Size Download File type/resource
GSM573681.CEL.gz 4.8 Mb (ftp)(http) CEL
GSM573681.CHP.gz 492.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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