|
| Status |
Public on Jul 29, 2011 |
| Title |
miRNA OSE 437 |
| Sample type |
RNA |
| |
|
| Source name |
Ovarian Surface Epithelia
|
| Organism |
Homo sapiens |
| Characteristics |
tumor stage/grade: N/A
age at surgery: 54
|
| Treatment protocol |
Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Affymetrix IVT Labeling Kit
|
| |
|
| Hybridization protocol |
Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
|
| Scan protocol |
as per manufacturer's protocol using GeneChip Scanner 3000.
|
| Description |
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
| Data processing |
MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
|
| |
|
| Submission date |
Aug 03, 2010 |
| Last update date |
Jul 29, 2011 |
| Contact name |
Shubin Shahab |
| E-mail(s) |
shubin.shahab@gmail.com
|
| Organization name |
Georgia Institute of Technology
|
| Department |
Biology
|
| Lab |
McDonald
|
| Street address |
310 Ferst Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30332 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE23391 |
miRNAs in ovarian cancer: A systems approach (mRNA data) |
| GSE23392 |
miRNAs in ovarian cancer: A systems approach |
|
