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Sample GSM573682 Query DataSets for GSM573682
Status Public on Jul 29, 2011
Title miRNA OSE 437
Sample type RNA
Source name Ovarian Surface Epithelia
Organism Homo sapiens
Characteristics tumor stage/grade: N/A
age at surgery: 54
Treatment protocol Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
Extracted molecule total RNA
Extraction protocol RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
Label biotin
Label protocol Affymetrix IVT Labeling Kit
Hybridization protocol Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
Scan protocol as per manufacturer's protocol using GeneChip Scanner 3000.
Description Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
Data processing MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
Submission date Aug 03, 2010
Last update date Jul 29, 2011
Contact name Shubin Shahab
Organization name Georgia Institute of Technology
Department Biology
Lab McDonald
Street address 310 Ferst Drive
City Atlanta
State/province GA
ZIP/Postal code 30332
Country USA
Platform ID GPL570
Series (2)
GSE23391 miRNAs in ovarian cancer: A systems approach (mRNA data)
GSE23392 miRNAs in ovarian cancer: A systems approach

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 830.79 P 0.0020226
AFFX-BioB-M_at 882.17 P 4.42873e-05
AFFX-BioB-3_at 765.16 P 6.02111e-05
AFFX-BioC-5_at 1666.41 P 4.42873e-05
AFFX-BioC-3_at 2433.87 P 4.42873e-05
AFFX-BioDn-5_at 4264.09 P 4.42873e-05
AFFX-BioDn-3_at 7638.37 P 0.000581214
AFFX-CreX-5_at 23938.6 P 4.42873e-05
AFFX-CreX-3_at 27202.1 P 4.42873e-05
AFFX-DapX-5_at 2.38632 A 0.996788
AFFX-DapX-M_at 67.9056 A 0.262827
AFFX-DapX-3_at 17.7167 A 0.712257
AFFX-LysX-5_at 87.0284 A 0.227636
AFFX-LysX-M_at 42.699 A 0.686277
AFFX-LysX-3_at 9.44838 A 0.843268
AFFX-PheX-5_at 3.87052 A 0.987453
AFFX-PheX-M_at 10.2909 A 0.981719
AFFX-PheX-3_at 81.5691 A 0.300606
AFFX-ThrX-5_at 8.41422 A 0.897835
AFFX-ThrX-M_at 53.5536 A 0.617401

Total number of rows: 54675

Table truncated, full table size 1619 Kbytes.

Supplementary file Size Download File type/resource
GSM573682.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM573682.CHP.gz 489.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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