|
| Status |
Public on Aug 04, 2011 |
| Title |
Human Embryonic Fibroblasts 24weeks |
| Sample type |
RNA |
| |
|
| Source name |
Fetal tissue 24 weeks
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: Human Embryonic Fibroblasts 24weeks
|
| Growth protocol |
Standard fibroblast medium, step liquid culture for FL and Fused cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Direct lysis of cells, DNAse treatment, RNeasy column purification
|
| Label |
Biotin
|
| Label protocol |
total RNA was labelled with a fluorophore using standard Affymetrix procedure (primer extension usig a polydT primer)
|
| |
|
| Hybridization protocol |
HuGene-1_0-st were hybridized with labelled probes using standard conditions recommended by the manufacturer
|
| Scan protocol |
GeneChip Scanner
|
| Description |
Gene expression data
|
| Data processing |
Data from CEL files were imported into RMA express software and normalized. The results were exported as log values.
|
| |
|
| Submission date |
Aug 04, 2010 |
| Last update date |
Aug 04, 2011 |
| Contact name |
Vladislav M Sandler |
| E-mail(s) |
vladislav.sandler@gmail.com
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Genetic Medicine
|
| Street address |
1300 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE23413 |
Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (HuGene-1_0-st) |
| GSE23416 |
Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells |
|
