|
| Status |
Public on Aug 04, 2011 |
| Title |
HEF24-SNP |
| Sample type |
genomic |
| |
|
| Source name |
Genomic DNA from Fibroblasts, 24 weeks
|
| Organism |
Homo sapiens |
| Characteristics |
sex: Female
cell type: Human Embryonic Fibroblasts 24weeks
|
| Growth protocol |
Standard medium for human fibroblasts.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNAeasy Qiagen
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
Data from CEL files were accessed using CLC Genomics Workbench 3. From all the SNPs detected by the array, we retained only the once located within exons, according to the hg18 genome annotation. This was done using Galaxy (http://main.g2.bx.psu.edu/)
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Aug 04, 2010 |
| Last update date |
Aug 04, 2011 |
| Contact name |
Vladislav M Sandler |
| E-mail(s) |
vladislav.sandler@gmail.com
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Genetic Medicine
|
| Street address |
1300 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE23414 |
Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells (GenomeWideSNP_6) |
| GSE23416 |
Reprogramming of Human Fibroblasts into Hematopoietic Progenitors by Fusion With Human Fetal Liver CD34+ Cells |
|
