|
| Status |
Public on Dec 12, 2022 |
| Title |
Early-onset B-cell lymphoma case X3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Early-onset precursor B-cell lymphoma by the irradiation of X-rays at 1 week of age
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
gender: Male
treatment: irradiation of X-rays at 1 week of age
disease state: Early-onset precursor B-cell lymphoma
tissue: B-cell lymphoma
|
| Treatment protocol |
Mice were left untreated or were subjected to whole-body irradiated with gamma-rays (4 Gy) or X-rays (3.8 Gy) at 1 or 7 weeks of age.
|
| Growth protocol |
Mice were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 3°C) and humidity (50 ± 10%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet and sterilized water ad libitum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA of B-cell lymphomas and normal normal ears were extarcted by AllPrep DNA/RNA/Protein Mini Kit and DNeasy Blood & Tissue Kit (Qiagen), respectively.
|
| Label |
Cy5
|
| Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| Channel 2 |
| Source name |
Normal ear
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
gender: Male
tissue: Ear skin
|
| Treatment protocol |
Mice were left untreated or were subjected to whole-body irradiated with gamma-rays (4 Gy) or X-rays (3.8 Gy) at 1 or 7 weeks of age.
|
| Growth protocol |
Mice were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 3°C) and humidity (50 ± 10%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet and sterilized water ad libitum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA of B-cell lymphomas and normal normal ears were extarcted by AllPrep DNA/RNA/Protein Mini Kit and DNeasy Blood & Tissue Kit (Qiagen), respectively.
|
| Label |
Cy3
|
| Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| |
| Hybridization protocol |
Slides were hybridised in 1X Agilent Blocking Agent/Hi-RPM Buffer for 24 hours at 67°C in a rotating hybridization oven, then washed with the Agilent Oligo aCGH Buffer 1 and Buffer 2 in an ozone-depleted hood.
|
| Scan protocol |
Scanned on an Agilent microarray scanner.
|
| Description |
Early-pBLX3
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| |
|
| Submission date |
Dec 22, 2021 |
| Last update date |
Dec 14, 2022 |
| Contact name |
Kazuhiro DAINO |
| E-mail(s) |
daino.kazuhiro@qst.go.jp
|
| Organization name |
National Institutes for Quantum and Radiological Science and Technology
|
| Street address |
4-9-1 Anagawa, Inage-ku
|
| City |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19767 |
| Series (1) |
| GSE192455 |
Genomic DNA copy number alterations of mouse B-cell lymphomas induced by ionizing radiation |
|
