|
Status |
Public on Apr 05, 2012 |
Title |
Huh7 CD133-, MAS5 |
Sample type |
RNA |
|
|
Source name |
Huh7 CD133-
|
Organism |
Homo sapiens |
Characteristics |
cell type: HCC cell line: Huh7
|
Growth protocol |
CD133+ and CD133- cells sorted from Huh7 HCC cells using flow cytometry cell sorting technique
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached) Ambion MessageAmp II-Biotin Enhanced (Cat #1791)
|
|
|
Hybridization protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached) According to Affymetrix protocol
|
Scan protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached) According to Affymetrix protocol
|
Description |
MAS5
|
Data processing |
The data were analyzed with MAS5 using GCOS Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Aug 06, 2010 |
Last update date |
Apr 05, 2012 |
Contact name |
Stephanie Ma |
E-mail(s) |
stefma@hku.hk
|
Organization name |
The University of Hong Kong
|
Department |
School of Biomedical Sciences
|
Street address |
Rm 47, 1/F, Lab Block, Faculty of Medicine Bldg, 21 Sassoon Rd, Pok Fu Lam
|
City |
Hong Kong |
ZIP/Postal code |
NA |
Country |
Hong Kong |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE23450 |
Expression data comparing Huh7 CD133+ and CD133- HCC cells |
|
Relations |
Reanalysis of |
GSM575349 |