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Sample GSM575347 Query DataSets for GSM575347
Status Public on Apr 05, 2012
Title Huh7 CD133-, MAS5
Sample type RNA
 
Source name Huh7 CD133-
Organism Homo sapiens
Characteristics cell type: HCC
cell line: Huh7
Growth protocol CD133+ and CD133- cells sorted from Huh7 HCC cells using flow cytometry cell sorting technique
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
Ambion MessageAmp II-Biotin Enhanced (Cat #1791)
 
Hybridization protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
According to Affymetrix protocol
Scan protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
According to Affymetrix protocol
Description MAS5
Data processing The data were analyzed with MAS5 using GCOS Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Aug 06, 2010
Last update date Apr 05, 2012
Contact name Stephanie Ma
E-mail(s) stefma@hku.hk
Organization name The University of Hong Kong
Department School of Biomedical Sciences
Street address Rm 47, 1/F, Lab Block, Faculty of Medicine Bldg, 21 Sassoon Rd, Pok Fu Lam
City Hong Kong
ZIP/Postal code NA
Country Hong Kong
 
Platform ID GPL570
Series (1)
GSE23450 Expression data comparing Huh7 CD133+ and CD133- HCC cells
Relations
Reanalysis of GSM575349

Data table header descriptions
ID_REF
VALUE MAS5 data
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 983.833 P 0.000389797
AFFX-BioB-M_at 1486.32 P 4.42873e-05
AFFX-BioB-3_at 843.869 P 6.02111e-05
AFFX-BioC-5_at 2630.56 P 5.16732e-05
AFFX-BioC-3_at 3514.61 P 4.42873e-05
AFFX-BioDn-5_at 5841.76 P 4.42873e-05
AFFX-BioDn-3_at 12734.8 P 7.00668e-05
AFFX-CreX-5_at 30960.4 P 5.16732e-05
AFFX-CreX-3_at 37559.5 P 4.42873e-05
AFFX-DapX-5_at 1228.58 P 6.02111e-05
AFFX-DapX-M_at 2346.88 P 0.000340305
AFFX-DapX-3_at 4175.65 P 5.16732e-05
AFFX-LysX-5_at 387.66 P 4.42873e-05
AFFX-LysX-M_at 307.604 P 0.000340305
AFFX-LysX-3_at 707.542 P 5.16732e-05
AFFX-PheX-5_at 480.643 P 5.16732e-05
AFFX-PheX-M_at 515.344 P 5.16732e-05
AFFX-PheX-3_at 451.501 P 4.42873e-05
AFFX-ThrX-5_at 281.715 P 0.00010954
AFFX-ThrX-M_at 427.686 P 5.16014e-05

Total number of rows: 54675

Table truncated, full table size 1638 Kbytes.




Supplementary file Size Download File type/resource
GSM575347_Huh7-CD133-ve.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM575347_Huh7-CD133-ve.MAS5.CHP.gz 303.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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