|
| Status |
Public on Apr 05, 2012 |
| Title |
Huh7 CD133-, RMA |
| Sample type |
RNA |
| |
|
| Source name |
Huh7 CD133-
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HCC
cell line: Huh7
|
| Growth protocol |
CD133+ and CD133- cells sorted from Huh7 HCC cells using flow cytometry cell sorting technique
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
Ambion MessageAmp II-Biotin Enhanced (Cat #1791)
|
| |
|
| Hybridization protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
According to Affymetrix protocol
|
| Scan protocol |
Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached)
According to Affymetrix protocol
|
| Description |
RMA
|
| Data processing |
The data were analyzed with RMA using GCOS Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 06, 2010 |
| Last update date |
Apr 05, 2012 |
| Contact name |
Stephanie Ma |
| E-mail(s) |
stefma@hku.hk
|
| Organization name |
The University of Hong Kong
|
| Department |
School of Biomedical Sciences
|
| Street address |
Rm 47, 1/F, Lab Block, Faculty of Medicine Bldg, 21 Sassoon Rd, Pok Fu Lam
|
| City |
Hong Kong |
| ZIP/Postal code |
NA |
| Country |
Hong Kong |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23450 |
Expression data comparing Huh7 CD133+ and CD133- HCC cells |
|
| Relations |
| Reanalyzed by |
GSM575347 |
