|
Status |
Public on May 30, 2012 |
Title |
Aurka_R_+Dox_rep2 |
Sample type |
RNA |
|
|
Source name |
Embryonic Stem Cell
|
Organism |
Mus musculus |
Characteristics |
cell line: AINV15 strain background: 129P2/OlaHsd genotype/variation: Aurka condition: +Dox
|
Treatment protocol |
Each rescue ES clone was grown in +Dox (1ug/ml) media; Dox withdrawal was performed for 5days
|
Growth protocol |
rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
3B
|
Data processing |
Data was VST transformed, RSN normalized in R using LUMI package
|
|
|
Submission date |
Aug 10, 2010 |
Last update date |
May 30, 2012 |
Contact name |
Yen-Sin Ang |
Organization name |
Gladstone Institute, UCSF
|
Street address |
1650 Owens Street
|
City |
san francisco |
State/province |
CALIFORNIA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE23541 |
Regulation of Embryonic and Induced Pluripotency by Aurora Kinase-p53 Signaling |
|