NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM577216 Query DataSets for GSM577216
Status Public on May 30, 2012
Title Aurka_R_-Dox_rep1
Sample type RNA
 
Source name Embryonic Stem Cell
Organism Mus musculus
Characteristics cell line: AINV15
strain background: 129P2/OlaHsd
genotype/variation: Aurka
condition: -Dox
Treatment protocol Each rescue ES clone was grown in +Dox (1ug/ml) media; Dox withdrawal was performed for 5days
Growth protocol rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
Extracted molecule total RNA
Extraction protocol total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 4A
Data processing Data was VST transformed, RSN normalized in R using LUMI package
 
Submission date Aug 10, 2010
Last update date May 30, 2012
Contact name Yen-Sin Ang
Organization name Gladstone Institute, UCSF
Street address 1650 Owens Street
City san francisco
State/province CALIFORNIA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6885
Series (1)
GSE23541 Regulation of Embryonic and Induced Pluripotency by Aurora Kinase-p53 Signaling

Data table header descriptions
ID_REF
VALUE RSN normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1377919 10.58132829 0
ILMN_2588051 13.3595872 0
ILMN_1377921 14.61291915 0
ILMN_2588053 14.40399147 0
ILMN_1377923 8.394362373 0
ILMN_2588055 11.49951094 0
ILMN_1377920 15.00889026 0
ILMN_2588052 15.06959728 0
ILMN_1377924 13.94693017 0
ILMN_2588056 14.78147331 0
ILMN_2622997 7.896103288 0.00125
ILMN_2416145 10.30213031 0
ILMN_2681195 7.50845398 0.66917
ILMN_2613422 7.547989213 0.4812
ILMN_2690349 7.634175011 0.16541
ILMN_2690348 7.602949851 0.24812
ILMN_1250088 7.505531406 0.68421
ILMN_2734060 10.63131665 0
ILMN_1244842 7.596857651 0.28195
ILMN_2506499 8.030546167 0

Total number of rows: 25697

Table truncated, full table size 765 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap