NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM5773737 Query DataSets for GSM5773737
Status Public on Feb 04, 2022
Title Control 2
Sample type SRA
 
Source name granulosa cells
Organism Homo sapiens
Characteristics tissue: ovary
cell type: granulosa cell
diagnosis: Healthy
Extracted molecule total RNA
Extraction protocol Human granulosa cells were separated with density gradient centrifugation and cultured in DMEM-F12 supplemented with 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin (5,000 U/mL) for 12h, and subsequently collected for RNA extraction.
A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description C2
Data processing Raw data (raw reads) of fastq format were firstly processed through in-house perl scripts. In this step, clean data (clean reads) were obtained by removing reads containing adapter, reads containing poly-N and low quality reads from raw data.
featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Genome_build: Homo_sapiens_Ensemble_98
Supplementary_files_format_and_content: gene_fpkm.txt: Matrix table with FPKM values for each Sample.
Supplementary_files_format_and_content: gene_count.txt: Matrix table with raw gene counts for every gene and every sample.
 
Submission date Jan 05, 2022
Last update date Feb 04, 2022
Contact name Yanli Pang
E-mail(s) yanlipang@bjmu.edu.cn
Phone +86-010-82266043
Organization name Reproductive Medicial Center , Peking University Third Hospital
Street address No.49 Huayuan-Bei Road , Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100191
Country China
 
Platform ID GPL24676
Series (1)
GSE193123 Effects of androgen excess-related metabolic disturbances on granulosa cell function and follicular development
Relations
BioSample SAMN24662001
SRA SRX13626906

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap