|
| Status |
Public on Nov 01, 2022 |
| Title |
12 weeks after cercarial infection |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Immune cell
agent: S. japonicum
time point: 12 weeks
strain: BALB/c
Sex: female
|
| Treatment protocol |
BALB/c mice were percutaneously infected with 30±2 cercariae of S. japonicum. Successful infection was confirmed by detecting serum specific antibody or identifying parasitic eggs in fecal samples after infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were sacrificed under anesthesia and spleens were harvested. Spleen tissue was minced in a sterile Petri plate. The erythrocytes were lysed in ACK buffer and a single-cell suspension of murine splenocytes was prepared by passing cells through 70 μm strainers.
Single cell RNA libraries were prepared using Chromium Single cell 5' Library & gel bead kit and coupled Chromium single cell V(D)J enrichment kit&scT/BCR library according to the manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
SC4
|
| Data processing |
Raw sequencing data were processed using the 10X Genomics Cell Ranger pipline
Genome_build: mm10 and GRCm38
Supplementary_files_format_and_content: Matrix tables with raw gene counts for every gene and cell ,TCR/BCR clonotype information
|
| |
|
| Submission date |
Jan 07, 2022 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Zhuo Yue |
| E-mail(s) |
ZhuoYue@tmu.edu.cn
|
| Organization name |
Tianjin medical university
|
| Street address |
Qixiangtai Road
|
| City |
Tianjin,China |
| ZIP/Postal code |
300000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE193216 |
Longitudinal Immune Profiling Highlights CD4+ T cell Exhaustion Correlated with Liver Fibrosis in Schistosoma japonicum Infection |
|
| Relations |
| BioSample |
SAMN24721972 |
| SRA |
SRX13655286 |
