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Sample GSM5821709 Query DataSets for GSM5821709
Status Public on Jan 20, 2022
Title Col-0 T0 poly rep1
Sample type SRA
 
Source name seedling
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
age: 5-d-old
treatment: 0 min with 150 mM NaCl
genotype: wildtype
Treatment protocol Seedlings were rested in 1 x MS liquid medium for 30 min and subjected to 1 x MS liquid medium supplie with 150 mM NaCl for 0 or 15 min
Growth protocol Sueface sterilized seeds were imbibed in water at 4 °C for 3 days and germinated on 1x MS agar at standard condition (16h ligth/8h dark, 22 °C) for 5 days
Extracted molecule total RNA
Extraction protocol Polysomal RNA were extracted from the mixed polysome fractions that were separated in a 10% to 50% sucrose gradient. Total RNA were extracted from each input lysate.
stander Illumina mRNA-seq protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Description polysomal mRNA
Data processing Bowtie for alignment, v2, default parameter
Cufflink for expression profiling, V2.1.0, default parameter
Genome_build: TAIR10
Supplementary_files_format_and_content: wzy_raw_data.xlsx
 
Submission date Jan 17, 2022
Last update date Jan 20, 2022
Contact name Bosheng Li
E-mail(s) libs@sustc.edu.cn
Organization name SUSTC (current, may different with previous institution )
Department MCDB
Street address SUStech Huiyuan #1 406
City Shenzhen
State/province Guangdong
ZIP/Postal code 581055
Country China
 
Platform ID GPL23157
Series (1)
GSE193804 Polysome profiling of Arabidopsis translatome upon hypertonic stress
Relations
BioSample SAMN25044034
SRA SRX13813901

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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