|
| Status |
Public on Jan 20, 2022 |
| Title |
Col-0 T15 poly rep1 |
| Sample type |
SRA |
| |
|
| Source name |
seedling
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
age: 5-d-old
treatment: 15 min with 150 mM NaCl
genotype: wildtype
|
| Treatment protocol |
Seedlings were rested in 1 x MS liquid medium for 30 min and subjected to 1 x MS liquid medium supplie with 150 mM NaCl for 0 or 15 min
|
| Growth protocol |
Sueface sterilized seeds were imbibed in water at 4 °C for 3 days and germinated on 1x MS agar at standard condition (16h ligth/8h dark, 22 °C) for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Polysomal RNA were extracted from the mixed polysome fractions that were separated in a 10% to 50% sucrose gradient. Total RNA were extracted from each input lysate.
stander Illumina mRNA-seq protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
polysomal mRNA
|
| Data processing |
Bowtie for alignment, v2, default parameter
Cufflink for expression profiling, V2.1.0, default parameter
Genome_build: TAIR10
Supplementary_files_format_and_content: wzy_raw_data.xlsx
|
| |
|
| Submission date |
Jan 17, 2022 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Bosheng Li |
| E-mail(s) |
libs@sustc.edu.cn
|
| Organization name |
SUSTC (current, may different with previous institution )
|
| Department |
MCDB
|
| Street address |
SUStech Huiyuan #1 406
|
| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
581055 |
| Country |
China |
| |
|
| Platform ID |
GPL23157 |
| Series (1) |
| GSE193804 |
Polysome profiling of Arabidopsis translatome upon hypertonic stress |
|
| Relations |
| BioSample |
SAMN25044028 |
| SRA |
SRX13813907 |
