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Sample GSM5834294 Query DataSets for GSM5834294
Status Public on Jan 24, 2025
Title Whole blood_Taxifolin_After_No4
Sample type RNA
 
Source name Human whole blood after taxifolin ingestion and cognitive measure test
Organism Homo sapiens
Characteristics Sex: Female
Treatment protocol Blood was collected using a PAX gene Blood RNA tube.
Extracted molecule total RNA
Extraction protocol Total RNA from whole blood was extracted using PAXgene Blood miRNA Kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated ss-cDNA were prepared according to the Thermo Fisher Scientific (Affymetrix) protocol from 50ng total RNA (Manual Target Preparation for GeneChip Whole Transctipt Expression Arrays, Publication Number MAN0018137, Revision B.0, 2020, Thermo Fisher Scientific).
 
Hybridization protocol Following fragmentation, 2.3 microgram of ss-cDNA were hybridized for 16 hr at 45C on Clariom S Array, human. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000 7G.
Data processing CEL files were quantified with the qFARMs, using the statistical language R (R-4.0.3) and Bioconductor 3.12
 
Submission date Jan 25, 2022
Last update date Jan 24, 2025
Contact name Fumika SHINOZAKI
E-mail(s) fp-shinozaki@newkast.or.jp
Organization name Kanagawa Institute of Industrial Science and Technology
Street address 3-25-13 Tonomachi, Kawasaki-ku
City Kawasaki
ZIP/Postal code 210-0821
Country Japan
 
Platform ID GPL23159
Series (1)
GSE194375 Acute effects of taxifolin food on whole blood gene expression levels after cognitive measure test.

Data table header descriptions
ID_REF
VALUE log2 qFARMs signal

Data table
ID_REF VALUE
23064070 8.563995008
23064071 6.842715047
23064072 6.55092803
23064073 6.733314351
23064074 5.912127015
23064075 8.486609507
23064076 6.40223397
23064077 7.574466545
23064078 7.458179359
23064079 6.399155154
23064080 12.11984699
23064081 5.609232778
23064083 5.820435409
23064084 5.148538993
23064085 5.941979512
23064086 5.356365511
23064087 6.470178758
23064088 6.068845739
23064089 5.096934275
23064090 6.693618469

Total number of rows: 27189

Table truncated, full table size 792 Kbytes.




Supplementary file Size Download File type/resource
GSM5834294_TA4.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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