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Sample GSM585199 Query DataSets for GSM585199
Status Public on Aug 20, 2010
Title Yeast-Ecoli control, Mnase
Sample type SRA
 
Source name Yeast-Ecoli mixture
Organisms Escherichia coli; Saccharomyces cerevisiae
Characteristics strain (yeast): BY4730 (MAT a, leu2∆0, met15∆0, ura3∆0)
strain (e. coli): JS5
Extracted molecule genomic DNA
Extraction protocol Genomic DNA from S.cerevisiae and E.coli was purified using Qiagen genomic tip 500/G, and mixed in a 3:1 mass ratio. The mixture was treated with MNase (USB) to yield a small average fragment size (< 300 bp), and DNA fragments of approximately 150 bp were purified by excision from an agarose gel.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing Mononucleosome size DNA fragments were sequenced using Illumina Genome Analyzer, and mapped to the S.cerevisiae genome (SGD April 2008 build) and to the E.coli K12 genome (U00096), allowing up to 2 mismatches per read.
Raw sequence read data are unavailable
 
Submission date Aug 19, 2010
Last update date Jun 11, 2013
Contact name George Locke
E-mail(s) glocke@physics.rutgers.edu
Phone (732) 445-3156
Fax (732) 445-5958
URL http://nucleosome.rutgers.edu/
Organization name Rutgers University
Department Physics
Street address 136 Frelinghuysen Rd
City Piscataway
State/province NJ
ZIP/Postal code 08854
Country USA
 
Platform ID GPL10839
Series (1)
GSE23712 High-throughput sequencing reveals a simple model of nucleosome energetics
Relations
BioSample SAMN02195988

Supplementary file Size Download File type/resource
GSM585199_FC14703_YE_MNase_Lane_1_eland_result.S.cerevisiae.reads.wig.gz 4.4 Mb (ftp)(http) WIG
GSM585199_FC14703_YE_MNase_Lane_1_eland_result.U00096.reads.wig.gz 650.2 Kb (ftp)(http) WIG
GSM585199_FC14703_YE_MNase_Lane_1_eland_result.txt.gz 115.5 Mb (ftp)(http) TXT
Processed data provided as supplementary file

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