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Sample GSM5855652 Query DataSets for GSM5855652
Status Public on Feb 08, 2022
Title Sir3myc_DSSY692_exp_Rep1
Sample type SRA
 
Source name haploid cells
Organism Saccharomyces cerevisiae
Characteristics strain: DSSY692/JRY13127
hm locus: HMLa::RFP
chip antibody: anti-myc
Extracted molecule genomic DNA
Extraction protocol Bead beating > Sonication > Clarification of lysate > ChIP with indicated antibody
NEB Ultra II kit
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina MiniSeq
 
Data processing Reads aligned to S288C reference genome (GenBank accession number GCA_000146045.2) using bowtie2
Coverage was computed with a custom Python script (Goodnight & Rine, 2021)
Coverage was normalized to the non-heterochromatin genome-wide median using a custom Python script (Goodnight & Rine, 2021)
Coverage was smoothed with a 300bp rolling mean in R
Genome_build: S288C (GenBank accession number GCA_000146045.2)
Supplementary_files_format_and_content: bedgraph
 
Submission date Feb 01, 2022
Last update date Feb 09, 2022
Contact name Daniel S Saxton
E-mail(s) dsaxton08@berkeley.edu
Phone 8314190756
Organization name University of California Berkeley
Department MCB
Lab Rine
Street address 100 University Ave
City Berkeley
State/province CA
ZIP/Postal code 94709
Country USA
 
Platform ID GPL22715
Series (1)
GSE195880 Distinct silencer states determine epigenetic states of heterochromatin
Relations
BioSample SAMN25555272
SRA SRX14006147

Supplementary file Size Download File type/resource
GSM5855652_Sir3myc_DSSY692_exp_Rep1.bedgraph.gz 66.5 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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