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Sample GSM589804 Query DataSets for GSM589804
Status Public on May 19, 2011
Title Luc control esiRNA, biological rep4
Sample type RNA
 
Source name R1/E ESC
Organism Mus musculus
Characteristics cell type: R1/E ESC
RNAi: Luciferase
Treatment protocol R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit protocol including DNaseI digest
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from mouse ESC after 48h RNAi against Luciferase
Data processing The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
 
Submission date Sep 01, 2010
Last update date May 19, 2011
Contact name Maciej Paszkowski-Rogacz
Organization name Technische Universit├Ąt Dresden
Department UCC / Medical Faculty and University Hospital Carl Gustav Carus
Lab Medical Systems Biology (Prof. Buchholz)
Street address Tatzberg 47/49
City Dresden
ZIP/Postal code 01307
Country Germany
 
Platform ID GPL1261
Series (2)
GSE23923 Expression data from Rad21 knock-down in ES cells
GSE24030 The Cohesin Complex Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity

Data table header descriptions
ID_REF
VALUE GC-RMA normalized signal intensity

Data table
ID_REF VALUE
1415670_at 11.5279
1415671_at 12.0723
1415672_at 12.7078
1415673_at 12.699
1415674_a_at 11.3495
1415675_at 9.73753
1415676_a_at 13.7092
1415677_at 8.5634
1415678_at 11.2688
1415679_at 12.1739
1415680_at 10.6696
1415681_at 11.3433
1415682_at 10.1224
1415683_at 13.1376
1415684_at 9.04878
1415685_at 11.2389
1415686_at 10.0811
1415687_a_at 10.107
1415688_at 11.873
1415689_s_at 8.26144

Total number of rows: 45101

Table truncated, full table size 849 Kbytes.




Supplementary file Size Download File type/resource
GSM589804.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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