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Sample GSM589807 Query DataSets for GSM589807
Status Public on May 19, 2011
Title Rad21 esiRNA, biological rep3
Sample type RNA
 
Source name R1/E ESC
Organism Mus musculus
Characteristics cell type: R1/E ESC
RNAi: Rad21
Treatment protocol R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit protocol including DNaseI digest
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from mouse ESC after 48h RNAi against Rad21
Data processing The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
 
Submission date Sep 01, 2010
Last update date May 19, 2011
Contact name Maciej Paszkowski-Rogacz
Organization name Technische Universit├Ąt Dresden
Department UCC / Medical Faculty and University Hospital Carl Gustav Carus
Lab Medical Systems Biology (Prof. Buchholz)
Street address Tatzberg 47/49
City Dresden
ZIP/Postal code 01307
Country Germany
 
Platform ID GPL1261
Series (2)
GSE23923 Expression data from Rad21 knock-down in ES cells
GSE24030 The Cohesin Complex Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity

Data table header descriptions
ID_REF
VALUE GC-RMA normalized signal intensity

Data table
ID_REF VALUE
1415670_at 11.3174
1415671_at 12.2515
1415672_at 12.5506
1415673_at 11.4752
1415674_a_at 11.1569
1415675_at 9.75037
1415676_a_at 13.657
1415677_at 8.14185
1415678_at 11.068
1415679_at 12.2826
1415680_at 10.5716
1415681_at 11.1957
1415682_at 10.1921
1415683_at 13.0195
1415684_at 9.29891
1415685_at 11.0807
1415686_at 9.92833
1415687_a_at 10.4319
1415688_at 11.7446
1415689_s_at 8.49546

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM589807.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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