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Sample GSM589891 Query DataSets for GSM589891
Status Public on Sep 02, 2010
Title prolactinoma treated with bromocriptine sample 2
Sample type RNA
 
Channel 1
Source name prolactinoma treated with bromocriptine
Organism Homo sapiens
Characteristics gender: male
age: 43y
tissue: prolactinoma
treatment: bromocriptine
Treatment protocol three of samples were treated with bromocriptine , no less than 5 mg per day was taken at least 8-week-long.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from pituitary adenoma tissues using Trizol Reagent (Invitrogen Life Technologies, USA) according to the manufacturer’s instructions with some modifications.
Label Cy3
Label protocol miRCURY LNA Array power labeling kit (Exiqon, Denmark)
 
Channel 2
Source name human prolactinoma
Organism Homo sapiens
Characteristics tissue: pool of all 6 treated and untreated prolactinomas
Treatment protocol three of samples were treated with bromocriptine , no less than 5 mg per day was taken at least 8-week-long.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from pituitary adenoma tissues using Trizol Reagent (Invitrogen Life Technologies, USA) according to the manufacturer’s instructions with some modifications.
Label Cy5
Label protocol miRCURY LNA Array power labeling kit (Exiqon, Denmark)
 
 
Hybridization protocol Exiqon protocol; The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere.
Scan protocol GenePix Pro 6.0.The microarray slides were scanned with GenePix 4000B Scanner.
Description reference (pool of sample 1-6)
Data processing The GPR file was extracted and exported to Genespring 7.3.1. The raw data were normalized using Data transformation: set measurements less than 0.01 to 0.01, per spot and per chip: Intensity dependent (Lowess) normalization, and per gene: normalize to median
 
Submission date Sep 01, 2010
Last update date Sep 01, 2010
Contact name wu zhe bao
E-mail(s) zhebaowu@yahoo.com.cn
Organization name First Affiliated Hospital of Wenzhou Medical College
Department Department of Neurosurgery
Street address west road of xueyuan
City wenzhou
ZIP/Postal code 325000
Country China
 
Platform ID GPL7722
Series (1)
GSE23927 microRNAs associated with bromocriptine-treated prolactinomas

Data table header descriptions
ID_REF
VALUE lowess normalized log2 ratio (test/reference).The ratio of green signal to red signal is calculated after background subtraction and normalization using the global Lowess (Locally Weighted Scatter plot Smoothing) regression algorithm (MIDAS, TIGR Microarray Data Analysis system)

Data table
ID_REF VALUE
3980
4040
4610
4700
5250
5560 0.233341102
5730
5740
6880 0.216791731
10138
10306
10482
10618 0.168623693
10919 0.20506234
10923
10925
10928 0.245069303
10936 1.04593479
10937
10942

Total number of rows: 856

Table truncated, full table size 8 Kbytes.




Supplementary file Size Download File type/resource
GSM589891_slide2.gpr.gz 406.8 Kb (ftp)(http) GPR
Processed data included within Sample table

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