NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM590441 Query DataSets for GSM590441
Status Public on Feb 21, 2011
Title Transgenic ER-FUS3, 36h, ESTR, 1
Sample type RNA
 
Channel 1
Source name ER-FUS3 transgenic plant (Col-0), untreated
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: no treatment
treatment time: 0h
replicate: 1 of 2
Growth protocol Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
Label Cy3
Label protocol Agilent Low RNA Input Linear Amplification Kit.
 
Channel 2
Source name ER-FUS3 transgenic plant (Col-0), ESTR, 36h
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: 4μM estrogen (ESTR)
treatment time: 36h
replicate: 1 of 2
Growth protocol Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
Label Cy5
Label protocol Agilent Low RNA Input Linear Amplification Kit.
 
 
Hybridization protocol Agilent Arabidopsis2 oligo microarray hybridization protocol.
Scan protocol Dual-laser Microarray scanner Agilent Technologies.
Description ER-FUS3 transgenic plants were used to identify the genes up-regulated in young seedlings of Arabidopsis in response to ectopic expression of FUS3.
Data processing Agilent Feature Extraction software version A.6.1.1.
 
Submission date Sep 03, 2010
Last update date Feb 21, 2011
Contact name Akiko Yamamoto
E-mail(s) yamamoto@agr.nagoya-u.ac.jp
Organization name Nagoya University
Department Bioscience and Biotechnology Center
Lab Laboratory of Plant Cell Function
Street address Furo-cho
City Nagoya
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL888
Series (1)
GSE23974 Gene regulation by the Arabidopsis seed maturation master regulator FUS3

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -1.05389
2 -0.0829471
3 -0.0875057
4 0.00665616
5 -0.184452
6 -0.113944
7 -1.12727
8 -0.00528557
9 0.26879
10 -0.134089
11 -0.0271553
12 -0.132285
13 0.0970161
14 -1.17248
15 -0.0699614
16 -0.0304001
17 -0.0157085
18 -0.03348
19 0.0753275
20 0.0507646

Total number of rows: 22575

Table truncated, full table size 342 Kbytes.




Supplementary file Size Download File type/resource
GSM590441.txt.gz 3.8 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap