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Sample GSM590449 Query DataSets for GSM590449
Status Public on Feb 21, 2011
Title Transgenic ER-FUS3, 3h, ESTR ABA, 1
Sample type RNA
 
Channel 1
Source name ER-FUS3 transgenic plant (Col-0), untreated
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: no treatment
treatment time: 0h
replicate: 1 of 2
Growth protocol Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
Label Cy3
Label protocol Agilent Low RNA Input Linear Amplification Kit.
 
Channel 2
Source name ER-FUS3 transgenic plant (Col-0), ESTR and ABA, 3h
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: 4μM estrogen (ESTR) & 100μM abscisic acid (ABA)
treatment time: 3h
replicate: 1 of 2
Growth protocol Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
Label Cy5
Label protocol Agilent Low RNA Input Linear Amplification Kit.
 
 
Hybridization protocol Agilent Arabidopsis2 oligo microarray hybridization protocol.
Scan protocol Dual-laser Microarray scanner Agilent Technologies.
Description ER-FUS3 transgenic plants were used to identify the genes up-regulated in young seedlings of Arabidopsis in response to ectopic expression of FUS3.
Data processing Agilent Feature Extraction software version A.6.1.1.
 
Submission date Sep 03, 2010
Last update date Feb 21, 2011
Contact name Akiko Yamamoto
E-mail(s) yamamoto@agr.nagoya-u.ac.jp
Organization name Nagoya University
Department Bioscience and Biotechnology Center
Lab Laboratory of Plant Cell Function
Street address Furo-cho
City Nagoya
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL888
Series (1)
GSE23974 Gene regulation by the Arabidopsis seed maturation master regulator FUS3

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -1.21712
2 0
3 -0.0443496
4 0.0388339
5 -0.158274
6 -0.351314
7 -1.31841
8 0.0928301
9 0.0430613
10 -0.532958
11 -0.0477183
12 0.0329702
13 0.0791273
14 -1.29974
15 -0.223356
16 0.0456654
17 -0.0162607
18 0.0991887
19 0.169505
20 0.0510819

Total number of rows: 22575

Table truncated, full table size 337 Kbytes.




Supplementary file Size Download File type/resource
GSM590449.txt.gz 3.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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