|
Status |
Public on Feb 21, 2011 |
Title |
Transgenic ER-FUS3, 3h, ESTR ABA, 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
ER-FUS3 transgenic plant (Col-0), untreated
|
Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Col-0 genotype: ER-FUS3 transgenic tissue: seedling age: 7 days treatment: no treatment treatment time: 0h replicate: 1 of 2
|
Growth protocol |
Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input Linear Amplification Kit.
|
|
|
Channel 2 |
Source name |
ER-FUS3 transgenic plant (Col-0), ESTR and ABA, 3h
|
Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Col-0 genotype: ER-FUS3 transgenic tissue: seedling age: 7 days treatment: 4μM estrogen (ESTR) & 100μM abscisic acid (ABA) treatment time: 3h replicate: 1 of 2
|
Growth protocol |
Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
|
Label |
Cy5
|
Label protocol |
Agilent Low RNA Input Linear Amplification Kit.
|
|
|
|
Hybridization protocol |
Agilent Arabidopsis2 oligo microarray hybridization protocol.
|
Scan protocol |
Dual-laser Microarray scanner Agilent Technologies.
|
Description |
ER-FUS3 transgenic plants were used to identify the genes up-regulated in young seedlings of Arabidopsis in response to ectopic expression of FUS3.
|
Data processing |
Agilent Feature Extraction software version A.6.1.1.
|
|
|
Submission date |
Sep 03, 2010 |
Last update date |
Feb 21, 2011 |
Contact name |
Akiko Yamamoto |
E-mail(s) |
yamamoto@agr.nagoya-u.ac.jp
|
Organization name |
Nagoya University
|
Department |
Bioscience and Biotechnology Center
|
Lab |
Laboratory of Plant Cell Function
|
Street address |
Furo-cho
|
City |
Nagoya |
ZIP/Postal code |
464-8601 |
Country |
Japan |
|
|
Platform ID |
GPL888 |
Series (1) |
GSE23974 |
Gene regulation by the Arabidopsis seed maturation master regulator FUS3 |
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