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Sample GSM591209 Query DataSets for GSM591209
Status Public on Dec 22, 2010
Title BM_CMP_BM_NBM1444
Sample type RNA
 
Source name bone marrow
Organism Homo sapiens
Characteristics patient: NBM1444
age group: adult
disease state: normal
cell source: bone marrow
cell type (purified cell subset): Lin-CD34+CD38+CD123+CD45RA-
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent containing either glycogen or linear acrylamide according to the manufacturer's protocol, and then treated with DNaseI (Ambion).
Label biotin
Label protocol Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
Scan protocol Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
Description BM_CMP_025.CEL
Data processing Raw CEL intensity data were normalized using MAS5
 
Submission date Sep 07, 2010
Last update date Dec 22, 2010
Contact name Ash A Alizadeh
E-mail(s) arasha@stanford.edu
Organization name Stanford University School of Medicine
Department Medicine, Divisions of Hematology and of Oncology
Lab Alizadeh
Street address 269 Campus Drive, CCSR 1145c
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL10881
Series (1)
GSE24006 A Leukemic Stem Cell Expression Signature is Associated with Clinical Outcomes in Acute Myeloid Leukemia

Data table header descriptions
ID_REF
VALUE normalized MAS5 signal intensities

Data table
ID_REF VALUE
AFFX-BioB-3_at 2376.095416
AFFX-BioB-5_at 2285.014279
AFFX-BioB-M_at 4001.912408
AFFX-BioC-3_at 3231.798677
AFFX-BioC-5_at 2692.796758
AFFX-BioDn-3_at 34822.22797
AFFX-BioDn-5_at 22714.00331
AFFX-CreX-3_at 104217.5864
AFFX-CreX-5_at 99767.38677
AFFX-DapX-3_at 162386.4608
AFFX-DapX-5_at 5533.111242
AFFX-DapX-M_at 56848.71542
AFFX-HSAC07/X00351_3_at 30021.04001
AFFX-HSAC07/X00351_5_at 552.3282896
AFFX-HSAC07/X00351_M_at 1946.890687
AFFX-hum_alu_at 216638.0498
AFFX-HUMGAPDH/M33197_3_at 36563.68219
AFFX-HUMGAPDH/M33197_5_at 1890.560888
AFFX-HUMGAPDH/M33197_M_at 7317.135977
AFFX-HUMISGF3A/M97935_3_at 900.7118765

Total number of rows: 29398

Table truncated, full table size 737 Kbytes.




Supplementary file Size Download File type/resource
GSM591209.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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