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Sample GSM591214 Query DataSets for GSM591214
Status Public on Dec 22, 2010
Title BM_GMP_BM_NBM1481
Sample type RNA
 
Source name bone marrow
Organism Homo sapiens
Characteristics patient: NBM1481
age group: adult
disease state: normal
cell source: bone marrow
cell type (purified cell subset): Lin-CD34+CD38+CD123+CD45RA+
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent containing either glycogen or linear acrylamide according to the manufacturer's protocol, and then treated with DNaseI (Ambion).
Label biotin
Label protocol Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
Scan protocol Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
Description BM_GMP_030.CEL
Data processing Raw CEL intensity data were normalized using MAS5
 
Submission date Sep 07, 2010
Last update date Dec 22, 2010
Contact name Ash A Alizadeh
E-mail(s) arasha@stanford.edu
Organization name Stanford University School of Medicine
Department Medicine, Divisions of Hematology and of Oncology
Lab Alizadeh
Street address 269 Campus Drive, CCSR 1145c
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL10881
Series (1)
GSE24006 A Leukemic Stem Cell Expression Signature is Associated with Clinical Outcomes in Acute Myeloid Leukemia

Data table header descriptions
ID_REF
VALUE normalized MAS5 signal intensities

Data table
ID_REF VALUE
AFFX-BioB-3_at 5933.118856
AFFX-BioB-5_at 7250.589971
AFFX-BioB-M_at 9789.733724
AFFX-BioC-3_at 10219.25021
AFFX-BioC-5_at 8856.45657
AFFX-BioDn-3_at 151218.8848
AFFX-BioDn-5_at 77213.31655
AFFX-CreX-3_at 383989.268
AFFX-CreX-5_at 345541.0318
AFFX-DapX-3_at 491228.3959
AFFX-DapX-5_at 173431.5087
AFFX-DapX-M_at 456065.2356
AFFX-HSAC07/X00351_3_at 42768.75722
AFFX-HSAC07/X00351_5_at 545.0026049
AFFX-HSAC07/X00351_M_at 3167.581284
AFFX-hum_alu_at 451348.191
AFFX-HUMGAPDH/M33197_3_at 57783.82106
AFFX-HUMGAPDH/M33197_5_at 2622.907976
AFFX-HUMGAPDH/M33197_M_at 13006.63267
AFFX-HUMISGF3A/M97935_3_at 1089.351449

Total number of rows: 29398

Table truncated, full table size 737 Kbytes.




Supplementary file Size Download File type/resource
GSM591214.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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