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Sample GSM591220 Query DataSets for GSM591220
Status Public on Dec 22, 2010
Title BM_HSC_BM_NBM1444
Sample type RNA
 
Source name bone marrow
Organism Homo sapiens
Characteristics patient: NBM1444
age group: adult
disease state: normal
cell source: bone marrow
cell type (purified cell subset): Lin-CD34+CD38-CD90+CD45RA-
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent containing either glycogen or linear acrylamide according to the manufacturer's protocol, and then treated with DNaseI (Ambion).
Label biotin
Label protocol Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
Scan protocol Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
Description BM_HSC_004.CEL
Data processing Raw CEL intensity data were normalized using MAS5
 
Submission date Sep 07, 2010
Last update date Dec 22, 2010
Contact name Ash A Alizadeh
E-mail(s) arasha@stanford.edu
Organization name Stanford University School of Medicine
Department Medicine, Divisions of Hematology and of Oncology
Lab Alizadeh
Street address 269 Campus Drive, CCSR 1145c
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL10881
Series (1)
GSE24006 A Leukemic Stem Cell Expression Signature is Associated with Clinical Outcomes in Acute Myeloid Leukemia

Data table header descriptions
ID_REF
VALUE normalized MAS5 signal intensities

Data table
ID_REF VALUE
AFFX-BioB-3_at 977.5588317
AFFX-BioB-5_at 1069.030155
AFFX-BioB-M_at 1479.091563
AFFX-BioC-3_at 2654.818341
AFFX-BioC-5_at 2315.386584
AFFX-BioDn-3_at 13731.50475
AFFX-BioDn-5_at 8174.975904
AFFX-CreX-3_at 47183.88842
AFFX-CreX-5_at 40830.38816
AFFX-DapX-3_at 38318.84854
AFFX-DapX-5_at 500.3126591
AFFX-DapX-M_at 9226.936462
AFFX-HSAC07/X00351_3_at 13234.55218
AFFX-HSAC07/X00351_5_at 355.5353004
AFFX-HSAC07/X00351_M_at 1189.481078
AFFX-hum_alu_at 79130.26427
AFFX-HUMGAPDH/M33197_3_at 31836.44492
AFFX-HUMGAPDH/M33197_5_at 3546.017012
AFFX-HUMGAPDH/M33197_M_at 10128.56241
AFFX-HUMISGF3A/M97935_3_at 2469.71888

Total number of rows: 29398

Table truncated, full table size 737 Kbytes.




Supplementary file Size Download File type/resource
GSM591220.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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