|Public on Sep 30, 2023
|2.3yo Female High hyperdiploid Acute Lymphoblastic Leukemia Patient_10
|WBC from Bone Marrow
|subject id: Patient_10
disease: B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL)
|treatment is according to IciCLe protocol of InPOG
|DNA extracted from mononuclear cell from Bone Marrow in EB at -20C
|Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from bone marrow at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, Qubit and agarose gel electrophoresis.
|As per manufacturer (Affymetrix)
|DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|hybridized to Affymetrix CytoScan HD
|The array image was acquired using Affymetrix GeneChip® Operating Software (GCOS version 1.4). Copy number values for individual SNPs were extracted and converted from CEL files into signal intensities using ChAS v1.5.
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|Mar 03, 2022
|Last update date
|Sep 30, 2023
|Tata Medical Center
|Cytogenetics and Genomics
|14 Major Arterial Road (E-W)
|Affymetrix SNP array data for acute lymphoblastic leukemia samples